FUNCTIONAL ACTIVITY OF HEMOPOIETIC PROGENITOR CELLS FROM CORD BLOOD IN LONG-TERM CULTURE ON HYDROGEL SUBSTRATE EX VIVO

Development of new and improvement existing models long-term cultivation hematopoietic stem cells (HSC) cord blood progenitor is one the most important directions in modern cell biology, related to obtaining a sufficient amount tissue for experimental clinical use. The aim work was investigate functional activity from during on soft polyacrylamide gel substrate presence cytokine complex determine advantages this method maintaining hematopoiesis further use transplantation. To realize purpose, following methods were used: vitro hydrogel substrate, colony formation semi-liquid agar, cytological research methods, light inverted microscopy, statistical methods. Due together with complex, high proliferative observed, which reflected their expansion (5 weeks), as well colony-forming (521.5 ± 7.5.105 per explanted cells). Thus, stiffness must be taken into account immediate descendants. made along cytokines ensures due long- term support hematopoiesis.